Package: primer3 (1.1.4-1) [universe]
Links for primer3
Download Source Package primer3:
Original Maintainers (usually from Debian):
- Debian-Med Packaging Team (Mail Archive)
- Steffen Moeller
- Charles Plessy
- David Paleino
It should generally not be necessary for users to contact the original maintainer.
- Homepage [primer3.sourceforge.net]
Tool to design flanking oligo nucleotides for DNA amplification
Primer3 picks primers for Polymerase Chain Reactions (PCRs), considering as criteria oligonucleotide melting temperature, size, GC content and primer-dimer possibilities, PCR product size, positional constraints within the source sequence, and miscellaneous other constraints. All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.
It has been published in Rozen S and Skaletsky H, "Primer3 on the WWW for general users and for biologist programmers.", Methods Mol Biol. 2000;132:365-86.
The Whitehead Institute for Biomedical Research provides a web-based front end to Primer3.
Other Packages Related to primer3
- sug: ncbi-epcr
- Tool to test a DNA sequence for the presence of sequence tagged sites
- enh: emboss
- the european molecular biology open software suite